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Blue light is part of the visible light spectrum that does not generate harmful DNA adducts associated with skin cancer and photoaging and may represent a safer therapeutic modality for treatment of keloid scars and other fibrotic skin diseases Our laboratory previously demonstrated that light-emitting diode LED red and infrared light inhibits proliferation of skin fibroblasts Moreover different wavelengths of light can produce different biological effects Furthermore the effects of LED blue light LED-BL on human skin fibroblasts are not well characterized This study investigated the effects of LED-BL on human skin fibroblast proliferation viability migration speed and reactive oxygen-species ROS generation Irradiation of adult human skin fibroblasts using commercially-available LED-BL panels was performed in vitro and modulation of proliferation and viability was quantified using the trypan blue dye exclusion assay migratory speed was assessed using time-lapse video microscopy and intracellular ROS generation was measured using the dihydrorhodamine flow cytometry assay Statistical differences between groups were determined by ANOVA and Student s t-test Human skin fibroblasts treated with LED-BL fluences of 5 10 15 30 and 80 J cm 2 demonstrated statistically significant dose-dependent decreases in relative proliferation of 8 4 29 1 33 8 51 7 and 55 1 respectively compared to temperature and environment matched bench control plates respectively LED-BL fluences of 5 30 45 and 80 J cm 2 decreased fibroblast migration speed to 95 7 0 P 0 64 81 3 5 5 P 0 021 48 5 2 7 P 0 0001 and 32 3 1 9 P 0 0001 respectively relative to matched controls LED fluences of 5 10 30 and 80 J cm 2 resulted in statistically significant increases in reactive oxygen species of 110 4 116 6 127 5 and 130 respectively relative to bench controls At the fluences studied LED-BL can inhibit adult human skin dermal fibroblast proliferation and migration speed and is associated with increased reactive oxygen species generation in a dose-dependent manner without altering viability LED-BL has the potential to contribute to the treatment of keloids and other fibrotic skin diseases and is worthy of further translational and clinical investigation 2015 Wiley Periodicals Inc

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Light emitting diode-generated blue light modulates fibrosis characteristics: fibroblast proliferation, migration speed,...